Genetic engineering in model organisms

12 June 2017 - 14 June 2017

Course language: English

The course consists of two parts: it starts with a three days' course (12-14 June) and next a two days' symposium (15 and 16 June). Both parts are obliged to attend.

The ability to modify genes at the level of the germ line by transgenesis and gene targeting has been crucial for our understanding of gene function and has yielded many highly valuable models for human diseases. This course will deal with the basic principles underlying the generation of transgenic, knock-out (KO) and knock-in (KI, i.e., gene-modified rather than gene-inactivated) mice.
Most recently, the spectacular advances in CRISPR/Cas9-assisted gene modification have found wide application in mouse genetics. CRISPR/Cas9-technology strongly facilitates gene targeting in ESCs, but can also directly be used in zygotes, evading the ESC route. Both applications will be presented, as well as the application of CRISPR/Cas9 technology in non-germ-line gene modification.

The course will consist of lectures covering technology and applications of genetic engineering in basic and medical research. Practical demonstrations will show crucial steps in the generation of transgenic, KO and KI mice, and principles of in vivo imaging.

The first three days of the course will prepare participants for the 2-day international workshop on Innovative Mouse Models (IMM 2017) immediately following the course. Keynote lectures from leading laboratories and presentations from selected abstracts will discuss the latest developments on advanced genome modification protocols and its applications in fundamental biology and biomedical research. Course participants will participate in ‘meet-the-expert’ sessions.

This introductory course is aimed at PhD students with little or no experience in the field of transgenesis and in vivo imaging, but is also of interest to those already working with animal models but wishing to expand their knowledge of the above technologies for applications in their own research. 

We only have facilities to accommodate 30 participants. Selection is on a first-come, first-served basis.

Deadline for registration 29 May 2017.

For more information: http://www.medgencentre.nl
 

Programme

For more information and an updated program please visit: www.medgencentre.nl

Start date

12 June 2017

Location

Leiden University Medical Center, building 1
Lecture hall 2
Albinusdreef 2
LEIDEN

Course organiser

Co-ordinator

Price

Participation is without costs for all members of MGC associated departments and OOA Amsterdam  
Course fee for other personnel of the LUMC or Erasmus MC € 400,00  
Course fee for participants outside of these institutes € 600,00  
 

Specification subscription

  • Participation is without costs for all members of MGC associated departments and OOA Amsterdam (3 days course and two days symposia including one dinner)
  • Course fee for other personnel of the LUMC or Erasmus MC (3 days course and two days symposia including one dinner)
  • Course fee for participants outside of these institutes (3 days course and two days symposia including one dinner)

Terms and conditions

To this course the general terms and conditions of Boerhaave CME are applicable.

Course specific cancellation terms

Deadline for registration is 29 May 2017. In case of cancellation after the deadline of registration or upon no show, participants from the MGC and OAA will be charged 50 euro; other participants have to pay the full price.